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Punnett’s Grid for Newsletter
			Parent 1 - D/+ - S/S - Sm/+
Parent 2 – Sm/Sm – S/+			D	D	"+"	"+"
			S	S	S	S
			Sm	"+"	Sm	"+"
"+"	S	Sm	D/+ S/S Sm/Sm	D/+ S/S Sm/+	"+/+ S/S Sm/Sm"	"+/+ S/S Sm/+"
	"+"	Sm	D/+ S/+ Sm/Sm	D/+ S/+ Sm/+	"+/+ S/+ Sm/Sm"	"+/+ S/+ Sm/+"

Compiled by Pterophyllum –  edited by Pete Bottle for publication

Angelfish 101 – Punnetts Square Competition

The competition from the last Newsletter to use the Punnetts square to predict the outcome of a cross between a Dark Smokey Turq (D/+ - S/S – Sm/+) turned out way more difficult than I imagined and completely stumped me and most of the entrants – and still does.

We need a tutorial on Punnetts square calculations with Multiple Alleles in each parent.

 

The entrants did a great job and I praise them for having the guts to try it.

However none actually met the criteria or results. Johan (Fish-Tail) came the closest but with a modified Punnetts grid and if further deliberation shows that it is technically correct then he will be awarded the prize retrospectively – sorry I don’t have an answer yet Johan.

Re-printing the results, by some, from a calculator display didn’t count – Chuckles – please take that in good humor.

In the end, Pterophyllum came to the rescue and has laid out a model grid (below) for us to digest and learn from.

Many thanks to Pterophyllum for giving us guidance.

If nothing else, this column is way overdue for those of us who want to increase our understanding of crosses which have multiple alleles.

Let me start by reinforcing what the requirements for the competition were,

1. To lay out a Punnetts Square Grid representing the relationships between the various alleles and,

2.  Correctly tabulate the resulting genotypes in the correct percentages.

 

The percentages that the genetics calculator produced for this cross are 8 separate genotypes in equal 12.5% portions, exactly as Pterophyllum has in his grid below.

 

Smokey Ghost                                                          +/+ - S/+ - Sm/+                     12.5%

Chocolate Ghost                                                      +/+ - S/+ - Sm/Sm                12.5%

Smokey Black Ghost                                              D/+ - S/+ - Sm/+                    12.5%

Chocolate Black Ghost                                          D/+ - S/+ - Sm/Sm                12.5%

Smokey Blushing                                                     +/+ - S/S – Sm/+                    12.5%

Chocolate Blushing                                                +/+ - S/S – Sm/Sm                12.5%

Smokey Turquoise Blushing                               D/+ - S/S - Sm/+                    12.5%

Chocolate Turquoise Blushing                            D/+ - S/S – Sm/Sm               12.5%

 

 

 

                                               

In my lack of understanding on this, my questions are

a) Why the 4 column by 3 row grid atop the main grid?

b) The process involved here with multiple alleles appears to be very different to a simple 4 square grid.

c) What process need to be used when accounting for crosses with fish that have multiple Alleles.

 

Deb and I had this pair in a tank and their offspring were beautiful fish, both parents are also veils. We have the female parent still but have her paired up with a Smokey Leopard Lace Veil– now their offspring are also stunning – another Punnetts square perhaps. The Smokey Leopard Lace must also carry a Stripeless gene in heterozygous form S/+ as many of their young are blushing

Pete Bottle